The 35-year-old defender, who came on within the 78th minute, had not played since the opening day.
Brescia played well till Florian Aye’s second yellow card for catching Aaron Ramsey on the edge of the box.
Paulo Dybala curled home the resulting free-kick and Juan Cuadrado clipped in an exceedingly second from Blaise Matuidi’s pass.
Juventus deserved their triumph, with Daniele Rugani’s header being tipped on to the bar by Serie A debutant Lorenzo Andrenacci, Rodrigo Bentancur hitting the post and Dybala crashing a trial off the crossbar in injury time.
Cristiano Ronaldo was rested as Juventus went back to the top of Serie A. inter Milan will reclaim top spot if they beat Lazio.